EAGLE (Environment And Genes in Lung cancer Etiology) is a large population-based case-control study designed and conducted to investigate the genetic and environmental determinants of lung cancer and smoking persistence using an integrative approach that allows combined analysis of genetic, environmental, clinical, and behavioral data. The study includes over 2,000 incident lung cancer cases, both males and females of Italian nationality, ages 35 to 79 years old, with verified lung cancer of any histological type, and over 2,000 healthy population-based controls matched to cases by age, gender, and residence.
Over 500,000 single nucleotide polymorphisms (SNPs) will be genotyped to identify genetic susceptibility to lung cancer risk and survival, and to smoking persistence in this project which was awarded funding by the National Human Genome Research Institute Genes, Environment and Health Initiative (GEI). After validation of a proportion of the significant SNPs in collaborative replication studies, further steps will include fine mapping of verified regions of interest, gene resequencing, and functional assays to provide biological clues for the findings. Analyses of smoking and other phenotypes across GEI-funded genome-wide association studies (GWAS) are planned.