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Reproducibility and Validity of Hormone Assays

In preparation for large-scale epidemiologic studies, Hormonal and Reproductive Epidemiology investigators have conducted a number of studies to assess methods for measurement of estrogens and estrogen metabolites. They have conducted formal proofs-of-performance to describe reliability of a recently developed liquid chromatography-tandem mass spectrometry (LC/MS2) method for measurement of 15 estrogens and estrogen metabolites in urine, and another to characterize the assay for use in measuring serum EM in unconjugated and total (conjugated + unconjugated) forms. Additional studies have documented the stability of urinary EM under varying conditions of processing and storage, and the comparability of LC/MS2 measures of urinary EM to previously employed immunoassays.

For more information, contact Gretchen Gierach.