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Reproducibility and Validity of Hormone Assays

In preparation for large-scale epidemiologic studies, Hormonal and Reproductive Epidemiology investigators have conducted a number of studies to assess methods for measurement of estrogens and estrogen metabolites. They have conducted formal proofs-of-performance to describe reliability of a recently developed liquid chromatography-tandem mass spectrometry (LC/MS-MS) method for measurement of 15 estrogens and estrogen metabolites in urine, and another to characterize the assay for use in measuring serum EM in unconjugated and total (conjugated + unconjugated) forms. Additional studies have documented the stability of urinary EM under varying conditions of processing and storage, and the comparability of LC/MS-MS measures of urinary EM to previously employed immunoassays. Recently, they conducted formal proofs-of-performance to describe the reproducibility of LC/MS-MS methods for measurement of 11 androgens and androgen metabolites in serum, as well as progesterone, pregnenolone, and progesterone metabolites in serum.

For more information, contact Gretchen Gierach and Britton Trabert.

Hormonal and Reproductive Epidemiology Branch - Research Areas