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Reproducibility and Validity of Hormone Assays

In preparation for large-scale epidemiologic studies, Hormonal and Reproductive Epidemiology investigators have conducted a number of studies to assess methods for measurement of estrogens and estrogen metabolites. They have conducted formal proofs-of-performance to describe reliability of a recently developed liquid chromatography-tandem mass spectrometry (LC/MS2) method for measurement of 15 estrogens and estrogen metabolites in urine (Falk RT, et al, Cancer Epidemiol Biomarkers Prev 2008, 17:3411), and another to characterize the assay for use in measuring serum EM in unconjugated and total (conjugated + unconjugated) forms (Fuhrman, unpublished). Additional studies have documented the stability of urinary EM under varying conditions of processing and storage (Fuhrman BJ, et al, Int J Biol Markers 2010;25:185) and the comparability of LC/MS2 measures of urinary EM to previously employed immunoassays (Faupel-Badger JM, et al, Cancer Epidemiol Biomarkers Prev 2010;19:292).

For more information, contact Gretchen Gierach.