In preparation for large-scale epidemiologic studies, investigators have conducted a number of studies to assess methods for measurement of estrogens and estrogen metabolites. They have conducted formal proofs-of-performance to describe reliability of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for measurement of 15 estrogens and estrogen metabolites in urine, and another to characterize the assay for use in measuring serum EM in unconjugated and total (conjugated + unconjugated) forms. Additional studies have documented the stability of urinary EM under varying conditions of processing and storage, and the comparability of LC-MS/MS measures of urinary EM to previously employed immunoassays.
Following the success of the estrogen metabolite assays, investigators conducted formal proofs-of-performance to describe the reproducibility of LC-MS/MS methods for measurement of 11 androgens and androgen metabolites in serum, as well as progesterone, pregnenolone, and progesterone metabolites in serum. Efforts are currently underway to validate assays in heparin and ethylenediaminetetraacetic acid (EDTA) plasma, and to estimate measures of temporal stability.
For more information, contact Britton Trabert.