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Reproducibility and Validity of Hormone Assays

In preparation for large-scale epidemiologic studies, investigators have conducted a number of studies to assess methods for measurement of estrogens and estrogen metabolites. They have conducted formal proofs-of-performance to describe reliability of a recently developed liquid chromatography-tandem mass spectrometry (LC/MS-MS) method for measurement of 15 estrogens and estrogen metabolites in urine, and another to characterize the assay for use in measuring serum EM in unconjugated and total (conjugated + unconjugated) forms. Additional studies have documented the stability of urinary EM under varying conditions of processing and storage, and the comparability of LC/MS-MS measures of urinary EM to previously employed immunoassays. Recently, they conducted formal proofs-of-performance to describe the reproducibility of LC/MS-MS methods for measurement of 11 androgens and androgen metabolites in serum, as well as progesterone, pregnenolone, and progesterone metabolites in serum.

For more information, contact Britton Trabert.

Metabolic Epidemiology Branch - Research Areas