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Molecular Epidemiology Research in OEEB

OEEB researchers incorporate state-of-the-art methods in biomarker analyses into many of their studies, which include cross-sectional, case-control, prospective, and case-series studies. This includes the use of both targeted and broad untargeted biomarker platforms (e.g., metabolomics, epigenomics, transcriptomics, adductomics, next generation sequencing) in a wide range of study designs to enhance exposure assessment, evaluate the biological plausibility of suggestive exposure-cancer associations, obtain mechanistic insights into the early biologic effects of established carcinogens, identify sources of susceptibility, and refine tumor subtyping at the molecular level to enhance analyses of exposure-cancer relationships.

Cross-sectional studies of occupational exposures and biomarker endpoints

Cross-sectional studies of healthy exposed and unexposed subjects using biomarker endpoints can identify exposures associated with key characteristics of carcinogens. These studies have been recently recognized by the International Agency for Research on Cancer (IARC) as playing an important role in cancer hazard identification, especially when epidemiologic studies of cancer are inconclusive, or infeasible, when biological plausibility of exposure-cancer associations is needed to help establish causality, or when the exposure of interest has been only recently introduced into the workplace and there has been inadequate latency to study cancer outcomes.

OEEB has a long-standing successful history of performing cross-sectional and short-term
longitudinal molecular epidemiology studies with a focus on organic chemicals (e.g., benzene, trichloroethylene), particulate matter (e.g., diesel exhaust, coal combustion, biomass combustion, e-Waste), pesticides, and biologically active dusts (e.g., endotoxin, glucans). These investigations are conducted in workplaces selected after extensive pilot work and site visits to characterize the work environment, and in study populations with well-characterized exposure histories. These studies generally enroll healthy individuals with a wide range of exposure levels and incorporate high quality, detailed subject-specific exposure data and measurements (air, dermal, and biological measures) that are important for gaining insight into exposure-response relationships and mechanisms of action of suspected and known carcinogens.

View examples of OEEB cross-sectional biomarker studies

Studies evaluating biomarkers linked to occupational and environmental exposures and future risk of cancer in prospective cohorts

OEEB has conducted a series of studies to evaluate the relationship between intermediate biomarkers (e.g., biomarkers of early biologic effect including telomere length, untargeted metabolomic-derived pathways, epigenetic changes, and immune and inflammatory markers) and risk of relevant cancers in prospective cohort studies. IARC has recognized the value of showing that biomarkers used in humans to evaluate the 10 key characteristics of cancer in cross-sectional studies are related to cancer risk as well as to exposure.

View examples of OEEB studies validating biomarkers of cancer risk

Genome Wide Association Studies in Case-Control and Cohort Studies and Evaluation of Gene-Environment Interactions 

OEEB has played a leadership role in large-scale, multi-center, international, genome-wide association studies (GWAS) and epigenome-wide association studies (EWAS), including studies of bladder cancer, kidney cancer, prostate cancer, lung cancer among never-smokers, and lymphoid neoplasms. Many of these studies capitalize on the high quality occupational and environmental exposure assessment in OEEB and extramural studies and DCEG expertise in genomics to evaluate gene-environment interactions.

View examples of OEEB genomic studies

Molecular pathology studies linking occupational and environmental carcinogenic exposures and tumor characteristics 

Molecular pathology studies conducted within OEEB integrate histological and molecular tumor tissue profiling with analyses of environmental and genetic risk factors to provide mechanistic insights into cancer etiology. These samples come from case-control studies or, at times, stand-alone case series. OEEB researchers have taken advantage of recent developments and efficiencies in targeted and whole genome DNA sequencing and RNA sequencing and expertise from additional Branches in DCEG to study bladder and kidney cancer tumors, and lung cancer tumors from never-smokers, to link molecular signatures to important exposures.

View examples of OEEB molecular pathology studies

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